Por favor utiliza este link para citar o compartir este documento: http://repositoriodigital.academica.mx/jspui/handle/987654321/378568
Título: Methodological aspects for the culture and quantification of heterotrophic sulfate-reducing bacteria
Palabras clave: Biología
hydrogen sulfide
sulfate-reducing bacteria
culture media
culture methods
SRB quantification
Editorial: Sociedad Venezolana de Microbiología
Descripción: This paper presents a comparison of various methods for culture, quantification, and maintenance of sulfate-reducing bacteria (SRB) under laboratory conditions, using liquid and semisolid media for water and soil samples. Starkey, Postgate B, API and modified Baars media were used with an incubation time of 21 days in a GasPack anaerobic jars type. The modified Baars medium was more efficient for the quantification of SRB in both liquid and semisolid media when compared with other culture media tested, detecting differences of three orders of magnitude in soil samples and in two orders for water samples at 8 days of incubation. The semisolid modified Baars medium in Petri dishes allowed the isolation of pure cultures of SRB by the streak plate method. It was found that strains in liquid modified Baars medium remain viable for up to three months, while in the same semisolid medium were kept only one month
Other Identifiers: http://www.redalyc.org/articulo.oa?id=199425417008
Aparece en las Colecciones:Revista de la Sociedad Venezolana de Microbiología

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